Degradation of the N-Heterocyclic Indole by the Pseudomonas putida strain mpky-1 Isolated from Subtropical Mangrove Sediment

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Karen Choi Yuen Yip, Ji-Dong Gu

Abstract


Biodegradation of indole by the Pseudomonas putida strain mpky-1 isolated and purified from sediment of the Inner Deep Bay of Hong Kong was investigated. The starin had 99.1% similarity with P. putida known. The biochemical degradation pathway of indole involved a hydroxylation reaction at the C-2 position first to form oxindole followed by a second hydroxylation at the C-3 position to isatin prior to the cleavage of the 5-carbon ring. This bacterium grew better at 22oC though it was capable of growth at low temperature (15oC in this case) with a longer lag phase was too long. The bacterial specific growth rate increased from 0.0035 hr-1 to 0.0249 hr-1 and the biodegradation rate also increased from 15oC to 30oC. P. putida mpky-1 grew quicker at pH 6.4 (specific growth rate, 0.0115 hr-1) than pH 7.4 (specific growth rate, 0.0066 hr-1) and pH 8.4 (specific growth rate, 0.036 hr-1) although the lag time of bacterial growth at pH 7.4 and pH 8.4 (15.01 hr and 15.00 hr, respectively) was very similar. The decrease in bacterial growth rate was also observed when salinity increased from 0.5% to 3.0%. P. putida mpky-1 may adapt to the Mai Po and Inner Deep Bay and degrade indole due to the pollution.

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DOI: http://dx.doi.org/10.26789/aeb.2017.01.001

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