Enhanced organic matter degradation by a sediment microbial fuel cell system using hexavalent chromium as an electron acceptor

VIEWS - 100 (Abstract) 8 (PDF)
Yongyan Niu, Khan Aman, Zhengjun Chen, Shuai Zhao, Ke-Jia Wu, Xingpeng Xiao, Xiangkai Li


In this study, a sediment microbial fuel cell (SMFC) system for the simultaneous biodegradation of organic matter and detoxification of hexavalent chromium Cr (VI) was investigated. The total organic carbon (TOC) removal rate of the SMFC with Cr (VI) was 30.07%, which was significantly higher than that in a SMFC without Cr (VI) (13.74%). In the SMFC with Cr (VI), the maximum values of open-circuit voltage (OCV) and power density were 408 mV and 4.8 mW/m2, respectively. During the long-term operation of the SMFC with Cr (VI), 25 mg/L of Cr (VI) were completely reduced from all four consecutive batches over 48 days. MiSeq sequencing revealed that the biofilm microbial community of the anode comprised of Bacteroidetes (42.9%), Proteobacteria (33.6%), Chloroflexi (7.5%), and Euryarchaeota (7.5%) as the predominant phyla. Compared with that of the sediment, certain families were enriched; they included Pseudomonadaceae (46.88-fold), Flavobacteriaceae (5.05-fold), and Syntrophaceae (4.48-fold), which are organic matter-degrading bacteria. These results suggest that SMFCs are useful for TOC removal and detoxification of heavy metals in remediation of contaminated lakes.


sediment microbial fuel cell, TOC removal, hexavalent chromium removal, detoxification, microbial communities

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Adelaja, O., Keshavarz, T., Kyazze, G. 2017. Treatment of phenan- threne and benzene using microbial fuel cells operated con- tinuously for possible in situ and ex situ applications. Inter- national Biodeterioration & Biodegradation, 116, 91-103. https://doi.org/10.1016/j.ibiod.2016.10.021

Arshad, M., Khan, A.H.A., Hussain, I., Badar-uz-Zaman, Anees, M., Iqbal, M., Soja, G., Linde, C., Yousaf, S. 2017. The reduction of chromium (VI) phytotoxicity and phytoavail- ability to wheat (Triticum aestivum L.) using biochar and bacteria. Applied Soil Ecology, 114, 90-98. https://doi.org/10.1016/j.apsoil.2017.02.021

Bond, D.R., Holmes, D.E., Tender, L.M., Lovley, D.R. 2002. Electrode-reducing microorganisms that harvest energy from marine sediments. Science, 295(5554), 483-485. https://doi.org/10.1126/science.1066771

Bond, D.R., Lovley, D.R. 2003. Electricity production by Geobac- ter sulfurreducens attached to electrodes. Applied and Envi- ronmental Microbiology, 69(3), 1548-1555. https://doi.org/10.1128/AEM.69.3.1548-1555.2003

Chaudhuri, S.K., Lovley, D.R. 2003. Electricity generation by direct oxidation of glucose in mediatorless microbial fuel cells. Nature Biotechnology, 21(10), 1229-1232. https://doi.org/ 10.1038/nbt867

Chovanec, P., Sparacino-Watkins, C.E., Zhang, N., Basu, P., Stolz,

J. 2012. Microbial reduction of chromate in the presence of nitrate by three nitrate respiring organisms. Frontiers in Microbiology, 3, 416. https://doi.org/10.3389/fmicb.2012.00416

Cruz, D.R., Santos, B.T., Cunha, G.C., Romaˆo, L.P. 2017. Green synthesis of a magnetic hybrid adsorbent (CoFe2O4/NOM):

Removal of chromium from industrial effluent and evaluation of the catalytic potential of recovered chromium ions. Journal of Hazardous Materials, 334, 76-85. https://doi.org/10.1016/j.jhazmat.2017.03.062

Fang, D., Zhang, X., Dong, M., Xue, X., 2017. A novel method to remove chromium, vanadium and ammonium from vana- dium industrial wastewater using a byproduct of magnesium- based wet flue gas desulfurization. Journal of Hazardous Materials,336, 8-20. https://doi.org/10.1016/j.jhazmat.2017.04.048

Gao, Y. and Xia, J., 2011. Chromium contamination accident in China: viewing environment policy of China. Environmental Science and Technology-Columbus, 45(20), 8605. https://www. dx.doi.org/10.1021/es203101f

Ge, Z., Zhang, F., Grimaud, J., Hurst, J. and He, Z., 2013. Long-term investigation of microbial fuel cells treating pri- mary sludge or digested sludge. Bioresource Technology, 136(136C), 509-514.


Gee, G.W. and Bauder, J.W., 1986. Particle-size analysis. pp. 383-

In: Klute A (ed), Methods of Soil Analysis: Part I (Second edition), Agronomy Monograph, vol. 9, ASA and SSSA, Madison, WI, USA.

Habibul, N., Hu, Y., Wang, Y.K., Chen, W., Yu, H.Q. and Sheng, G.P., 2016. Bioelectrochemical chromium (VI) removal in plant-microbial fuel cells. Environmental Science & Tech- nology, 50(7), 3882-3889.


Hong, S.W., Chang, I.S., Choi, Y.S. and Chung, T.H., 2009a. Ex- perimental evaluation of influential factors for electricity harvesting from sediment using microbial fuel cell. Biore- source Technology, 100(12), 3029-35. https://doi.org/10.1016/j.biortech.2009.01.030

Hong, S.W., Chang, I.S., Yongsu, C. and Taihak, C. 2009b. Ex- perimental evaluation of influential factors for electricity harvesting from sediment using microbial fuel cell. Biore- source Technology, 100(12), 3029-3035. https://doi.org/10.1016/j.biortech.2009.01.030

Huang, H., Wu, K., Khan, A., Jiang, Y., Ling, Z., Liu, P., Chen, Y., Tao, X. and Li, X., 2016. A novel Pseudomonas ges- sardii strain LZ-E simultaneously degrades naphthalene and reduces hexavalent chromium. Bioresource Technology, 207, 370-378.


Huang, L., Chai, X., Chen, G. and Logan, B.E., 2011. Effect of set potential on hexavalent chromium reduction and electricity generation from biocathode microbial fuel cells. Environ- mental Science & Technology, 45(11), 5025-5031. https://doi.org/10.1021/es103875d

Huang, L., Chen, J., Quan, X. and Yang, F., 2010. Enhancement of hexavalent chromium reduction and electricity produc- tion from a biocathode microbial fuel cell. Bioprocess and Biosystems Engineering, 33(8), 937-945. https://doi.org/10.1007/s00449-010-0417-7

Jayapriya, J. and Ramamurthy, V., 2012. Use of non-native phenazines to improve the performance of Pseudomonas aeruginosa MTCC 2474 catalysed fuel cells. Bioresource Technology, 124, 23-28. https://doi.org/10.1016/j.biortech.2012.08.034

Kim, B.H., Chang, I.S. and Gadd, G.M., 2007. Challenges in microbial fuel cell development and operation. Applied Mi- crobiology and Biotechnology, 76(3), 485-494. https://doi.org/10.1007/s00253-007-1027-4

Korak, J.A., Huggins, R. and Arias-Paic, M., 2017. Regener- ation of pilot-scale ion exchange columns for hexavalent chromium removal. Water Research, 118, 141-151. https://doi.org/10.1016/j.watres.2017.03.018

Li, W.W. and Yu, H.Q., 2015. Stimulating sediment bioreme- diation with benthic microbial fuel cells. Biotechnology Advances, 33(1), 1-12. https://www.dx.doi.org/10.1016/j.chemosphere.2006.10.074

Li, Z., Zhang, X. and Lei, L., 2008. Electricity production during the treatment of real electroplating wastewater containing

Cr6+ using microbial fuel cell. Process Biochemistry, 43(12),



Liu, G., Yang, H., Wang, J., Jin, R., Zhou, J. and Lv, H., 2010. Enhanced chromate reduction by resting Escherichia coli cells in the presence of quinone redox mediators. Bioresource Technology, 101(21), 8127-8131. https://doi.org/10.1016/j.biortech.2010.06.050

Lu, A., Zhong, S., Chen, J., Shi, J., Tang, J. and Lu, X., 2006. Removal of Cr(VI) and Cr(III) from aqueous solutions and industrial wastewaters by natural clino-pyrrhotite. Environ- mental Science & Technology, 40(9), 3064-3069. https://doi.org/ 10.1021/es052057x

Lu, L., Xing, D. and Ren, Z.J., 2015. Microbial community struc- ture accompanied with electricity production in a constructed wetland plant microbial fuel cell. Bioresource Technology, 195, 115-121.


Luo, J., Li, M., Zhou, M. and Hu, Y., 2015. Characterization of a novel strain phylogenetically related to Kocuria rhizophila and its chemical modification to improve performance of microbial fuel cells. Biosensors and Bioelectronics, 69, 113- 120.


Mahmoud, A.M. and El-Twab, S.M.A., 2017. Caffeic acid phenethyl ester protects the brain against hexavalent chromium toxicity by enhancing endogenous antioxi- dants and modulating the JAK/STAT signaling pathway. Biomedicine & Pharmacotherapy, 91, 303-311. https://www. dx.doi.org/ 10.1016/j.biopha.2017.04.073

Miao, Y., Liao, R., Zhang, X.-X., Wang, Y., Wang, Z., Shi, P., Liu, B. and Li, A., 2015. Metagenomic insights into Cr(VI) effect on microbial communities and functional genes of an expanded granular sludge bed reactor treating high-nitrate wastewater. Water Research, 76, 43-52. https://doi.org/10.1016/j.watres.2015.02.042

Pu, C., Addai, B., Pan, X. and Bo, P., 2017. Securitization product design for China’s environmental pollution liability insur- ance. Environmental Science and Pollution Research, 24(4), 3336-3351.

https://www. dx.doi.org/10.1007/s11356-016-8172-1

Qu, J. and Fan, M., 2010. The current state of water quality and technology development for water pollution control in China. Critical Reviews in Environmental Science and Technology, 40(6), 519-560.

https://www. dx.doi.org/10.1080/10643380802451953

Sethunathan, N. and Yoshida, T., 1973. A Flavobacterium sp.

that degrades diazinon and parathion. Canadian Journal of Microbiology, 19(7), 873-875.

https://doi.org/ 10.1139/m73-138

Shang, J., Zong, M., Yu, Y., Kong, X., Du, Q. and Liao, Q., 2017. Removal of chromium (VI) from water using nanoscale ze- rovalent iron particles supported on herb-residue biochar. Journal of Environmental Management, 197, 331e337. https://doi.org/10.1016/j.jenvman.2017.03.085

Sherafatmand, M. and Ng, H.Y., 2015. Using sediment microbial fuel cells (SMFCs) for bioremediation of polycyclic aromatichydrocarbons (PAHs). Bioresource Technology, 195, 122-130.


Tandukar, M., Huber, S.J., Onodera, T., Pavlostathis, S.G., 2009. Biological chromium (VI) reduction in the cathode of a microbial fuel cell. Environmental Science & Technology, 43(21), 8159-8165.


Thomas, F., Hehemann, J.H., Rebuffet, E., Czjzek, M. and Michel, G., 2011. Environmental and Gut Bacteroidetes: The Food Connection. Frontiers in Microbiology, 2(93), 93. https://doi.org/10.3389/fmicb.2011.00093

Wang, C., Chen, J., Hu, W.J., Liu, J.-Y., Zheng, H.L. and Zhao, F., 2014. Comparative proteomics reveal the impact of Om- cA/MtrC deletion on Shewanella oneidensis MR-1 in re- sponse to hexavalent chromium exposure. Applied Microbi- ology and Biotechnology, 98(23), 9735-9747. https://doi.org/10.1007/s00253-014-6143-3

Wang, G., Huang, L. and Zhang, Y., 2008. Cathodic reduction of hexavalent chromium [Cr(VI)] coupled with electricity generation in microbial fuel cells. Biotechnology Letters, 30(11), 1959.


Wang, H., Liu, D.M., Lu, L., Zhao, Z.W., Xu, Y.P. and Cui, F.Y.,

Degradation of algal organic matter using microbial fuel cells and its association with trihalomethane precursor removal. Bioresource Technology, 116(7), 80-85. https://doi.org/10.1016/j.biortech.2012.04.021

Wang, H., Qu, Y., Li, D., Zhou, X. and Feng, Y., 2015. Evaluation of an integrated continuous stirred microbial electrochemical reactor: Wastewater treatment, energy recovery and micro- bial community. Bioresource Technology, 195, 89-95. https://doi.org/10.1016/j.biortech.2015.06.039

Wu, W., Huang, H., Ling, Z., Yu, Z., Jiang, Y., Liu, P. and Li, X., 2016. Genome sequencing reveals mechanisms for heavy metal resistance and polycyclic aromatic hydrocarbon degra- dation in Delftia lacustris strain LZ-C. Ecotoxicology, 25(1), 234-247.


Xafenias, N., Zhang, Y. and Banks, C.J., 2013., Enhanced per- formance of hexavalent chromium reducing cathodes in the presence of Shewanella oneidensis MR-1 and lactate. Envi- ronmental Science & Technology, 47(9), 4512-4520. https://doi.org/10.1021/es304606u

Xafenias, N., Zhang, Y. and Banks, C.J., 2015. Evaluating hex- avalent chromium reduction and electricity production in microbial fuel cells with alkaline cathodes. International Journal of Environmental Science and Technology, 12(8), 2435-2446.

https://doi.org/ 10.1007/s13762-014-0651-7

Xu, B., Ge, Z. and He, Z., 2015. Sediment microbial fuel cells for wastewater treatment: challenges and opportunities. En- vironmental Science: Water Research & Technology, 1(3), 279-284.


Xu, J., Yu, Y., Wang, P., Guo, W., Dai, S. and Sun, H., 2007.

Polycyclic aromatic hydrocarbons in the surface sediments from Yellow River, China. Chemosphere, 67(7), 1408-1414. https://www. dx.doi.org/10.1016/j.chemosphere.2006.10.074

Xu, X., Zhao, Q. and Wu, M.S., 2015a. Removal and changes of sediment organic matter and electricity generation by sedi- ment microbial fuel cells and amorphous ferric hydroxide. Chemical and Biochemical Engineering Quarterly Journal,28(4), 561-566.


Xu, X., Zhao, Q. and Wu, M.S., 2015b. Improved biodegradation of total organic carbon and polychlorinated biphenyls for electricity generation by sediment microbial fuel cell and surfactant addition. RSC Advances, 5(77), 62534-62538. https://www. dx.doi.org/10.1039/C5RA12817J

Xu, X., Zhao, Q., Wu, M., Ding, J. and Zhang, W., 2017. Biodegra- dation of organic matter and anodic microbial communities analysis in sediment microbial fuel cells with/without Fe(III) oxide addition. Bioresource Technology, 225, 402-408. https://doi.org/10.1016/j.biortech.2016.11.126

Yan, Z., Song, N., Cai, H., Tay, J.H. and Jiang, H., 2012. Enhanced degradation of phenanthrene and pyrene in freshwater sedi- ments by combined employment of sediment microbial fuel cell and amorphous ferric hydroxide. Journal of Hazardous Materials, 199, 217-225. https://doi.org/10.1016/j.jhazmat.2011.10.087

Yang, Y., Lu, Z., Lin, X., Xia, C., Sun, G., Lian, Y. and Xu, M., 2015. Enhancing the bioremediation by harvesting elec- tricity from the heavily contaminated sediments. BioresourTechnology, 179, 615-618. https://doi.org/10.1016/j.biortech.2014.12.034

You, S., Zhao, Q., Zhang, J., Jiang, J. and Zhao, S., 2006. A mi- crobial fuel cell using permanganate as the cathodic electron acceptor. Journal of Power Sources, 162(2), 1409-1415. https://doi.org/10.1016/j.jpowsour.2006.07.063

Zhang, G., Zhao, Q., Jiao, Y. and Lee, D.J., 2015. Long-term oper- ation of manure-microbial fuel cell. Bioresource Technology, 180, 365-369.


Zhang, T., Cui, C., Chen, S., Ai, X., Yang, H., Shen, P. and Peng, Z., 2006. A novel mediatorless microbial fuel cell based on direct biocatalysis of Escherichia coli. Chemical Communications, (21), 2257-2259. https://doi.org/10.1039/b600876c

Zhao, F., Rahunen, N., Varcoe, J.R., Chandra, A., Avignone-Rossa, C., Thumser, A.E. and Slade, R.C., 2008. Activated carbon cloth as anode for sulfate removal in a microbial fuel cell. Environmental Science & Technology, 42(13), 4971-4976. https://doi.org/10.1021/es8003766

DOI: http://dx.doi.org/10.26789/AEB.2018.01.001


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